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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
15/02/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
GUIMARÃES, F. F.; JOAQUIM, S. F.; MANZI, M. P.; SILVA, R. C. da; BRUDER-NASCIMENTO, A. C. M. de O.; COSTA, E. O.; LANGONI, H. |
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Afiliação: |
FELIPE FEITAS GUIMARÃES, NUPEMAS/FMVZ/UNESP; SAMEA FERNANDES JOAQUIM, NUPEMAS/FMVZ/UNESP; MARCELA PINHO MANZI, NUPEMAS/FMVZ/UNESP; RODRIGO COSTA DA SILVA, NUPEMAS/FMVZ/UNESP; ARIANE CRISTINA MENDES DE OLIVEIRA BRUDER-NASCIMENTO, NUPEMAS/FMVZ/UNESP; ELIZABETH OLIVEIRA COSTA, NAPGAMA/USP; HÉLIO LANGONI, NUPEMAS/FMVZ/UNESP. |
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Título: |
Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 12, p. 1155-1160-1164, dez. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
In addition to Staphylococcus aureus nowadays other coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS), earlier considered of minor importance, are now accepted as relevant pathogens for humans and animals. The involvement of these microorganisms in bovine mastitis etiology and the possibility their transmission through milk to humans justify the requirement of developing reliable methods for identification of the most frequent species among them. The purpose of this study was to compare the phenotypic techniques with the genotypic method carried out by sequencing of the rpoB gene in identification of several species of the genus Staphylococcus isolated from bovine mastitis. A total of 300 staphylococci isolates of bovine mastitis cases from several Brazilian dairy herds were studied by phenotypic and genotypic techniques, respectively: 150 CoPS and 150 CoNS strains. A total of 18 CoNS different species and 4 CoPS species were identified. Among the CoNS the following species were recognized: 48 (32%) Staphylococcus warneri, 22(15%) S. epidermidis, 20(13%) S. hyicus, 10(7%) S. xylosus, 7(5%) S. haemolyticus, 6(4%) S. simulans, 6(4%) S. schleiferi subsp schleiferi, 6(4%) S. hominis, 5(3%) S. pasteuri, 4(2.7%) S. cohnii, 3(2%) S. saprophyticus subsp. saprophyticus 3(2%) S. chromogenes 3(2%) S. sciuri, 2(1%) S. saccharolyticus, 2(1%) S. lugdunensi, 1(0,7%) S. auricularis, 1(70%) S. saprophyticus subsp. bovis, 1(0.7%) S. capitis. And among the 150 CoPS were identified respectively: 105 (70%) S. aureus, 21(14%), S. hyicus, 19(13%) S. intermedius e 5(3%) S. schleiferi subsp coagulans. Considering the 150 CoNS isolates, the identifications performed by phenotypic and genotypic tests presented 96.7% of concordance, kappa coefficient of agreement = 0.933, SE (standard error) of kappa=0.021 (95% confidence interval: 0.893 to 0.974), Pearson?s correlation coefficient (r) = 0.9977, (confidence interval 95%: 0.9938 a 0.9992) and in relation to 150 CPS isolates it was detected an agreement of 98.7%, kappa = 0.960, SE of kappa = 0.016,(95% confidence interval: 0.929 to 0.992) Pearson?s correlation coefficient (r) = 0.9994 (95% confidence interval: 0.9681 to 1.0000). The verified agreement strength between the identification methods can be considered as excellent. These results assure that according to laboratory resources any of them will be suitable to perform the staphylococci identification. MenosIn addition to Staphylococcus aureus nowadays other coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS), earlier considered of minor importance, are now accepted as relevant pathogens for humans and animals. The involvement of these microorganisms in bovine mastitis etiology and the possibility their transmission through milk to humans justify the requirement of developing reliable methods for identification of the most frequent species among them. The purpose of this study was to compare the phenotypic techniques with the genotypic method carried out by sequencing of the rpoB gene in identification of several species of the genus Staphylococcus isolated from bovine mastitis. A total of 300 staphylococci isolates of bovine mastitis cases from several Brazilian dairy herds were studied by phenotypic and genotypic techniques, respectively: 150 CoPS and 150 CoNS strains. A total of 18 CoNS different species and 4 CoPS species were identified. Among the CoNS the following species were recognized: 48 (32%) Staphylococcus warneri, 22(15%) S. epidermidis, 20(13%) S. hyicus, 10(7%) S. xylosus, 7(5%) S. haemolyticus, 6(4%) S. simulans, 6(4%) S. schleiferi subsp schleiferi, 6(4%) S. hominis, 5(3%) S. pasteuri, 4(2.7%) S. cohnii, 3(2%) S. saprophyticus subsp. saprophyticus 3(2%) S. chromogenes 3(2%) S. sciuri, 2(1%) S. saccharolyticus, 2(1%) S. lugdunensi, 1(0,7%) S. auricularis, 1(70%) S. saprophyticus subsp. bovis, 1(0.7%) S. capitis. And among the 150... Mostrar Tudo |
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Palavras-Chave: |
Biochemical tests; Coagulase positive staphylococcus; Sequencing rpoB gene. |
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Thesaurus Nal: |
Genotype; Mastitis; Phenotype. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155734/1/Comparison-phenotypic-and-genotypic.pdf
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Marc: |
LEADER 03301naa a2200265 a 4500
001 2064243
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aGUIMARÃES, F. F.
245 $aComparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis.$h[electronic resource]
260 $c2016
520 $aIn addition to Staphylococcus aureus nowadays other coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS), earlier considered of minor importance, are now accepted as relevant pathogens for humans and animals. The involvement of these microorganisms in bovine mastitis etiology and the possibility their transmission through milk to humans justify the requirement of developing reliable methods for identification of the most frequent species among them. The purpose of this study was to compare the phenotypic techniques with the genotypic method carried out by sequencing of the rpoB gene in identification of several species of the genus Staphylococcus isolated from bovine mastitis. A total of 300 staphylococci isolates of bovine mastitis cases from several Brazilian dairy herds were studied by phenotypic and genotypic techniques, respectively: 150 CoPS and 150 CoNS strains. A total of 18 CoNS different species and 4 CoPS species were identified. Among the CoNS the following species were recognized: 48 (32%) Staphylococcus warneri, 22(15%) S. epidermidis, 20(13%) S. hyicus, 10(7%) S. xylosus, 7(5%) S. haemolyticus, 6(4%) S. simulans, 6(4%) S. schleiferi subsp schleiferi, 6(4%) S. hominis, 5(3%) S. pasteuri, 4(2.7%) S. cohnii, 3(2%) S. saprophyticus subsp. saprophyticus 3(2%) S. chromogenes 3(2%) S. sciuri, 2(1%) S. saccharolyticus, 2(1%) S. lugdunensi, 1(0,7%) S. auricularis, 1(70%) S. saprophyticus subsp. bovis, 1(0.7%) S. capitis. And among the 150 CoPS were identified respectively: 105 (70%) S. aureus, 21(14%), S. hyicus, 19(13%) S. intermedius e 5(3%) S. schleiferi subsp coagulans. Considering the 150 CoNS isolates, the identifications performed by phenotypic and genotypic tests presented 96.7% of concordance, kappa coefficient of agreement = 0.933, SE (standard error) of kappa=0.021 (95% confidence interval: 0.893 to 0.974), Pearson?s correlation coefficient (r) = 0.9977, (confidence interval 95%: 0.9938 a 0.9992) and in relation to 150 CPS isolates it was detected an agreement of 98.7%, kappa = 0.960, SE of kappa = 0.016,(95% confidence interval: 0.929 to 0.992) Pearson?s correlation coefficient (r) = 0.9994 (95% confidence interval: 0.9681 to 1.0000). The verified agreement strength between the identification methods can be considered as excellent. These results assure that according to laboratory resources any of them will be suitable to perform the staphylococci identification.
650 $aGenotype
650 $aMastitis
650 $aPhenotype
653 $aBiochemical tests
653 $aCoagulase positive staphylococcus
653 $aSequencing rpoB gene
700 1 $aJOAQUIM, S. F.
700 1 $aMANZI, M. P.
700 1 $aSILVA, R. C. da
700 1 $aBRUDER-NASCIMENTO, A. C. M. de O.
700 1 $aCOSTA, E. O.
700 1 $aLANGONI, H.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 12, p. 1155-1160-1164, dez. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 5 | |
| 1. |  | LANGONI, H.; GUIMARÃES, F. F.; COSTA, E. O. da; JOAQUIM, S. F.; MENOZZI, B. D. Celularidade do leite e unidades formadoras de colônias nas mastites causadas por Staphylococcus coagulase positiva e coagulase negativa. Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 6, p. 518-524, jun. 2015| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | LANGONI, H.; SALINA, A.; OLIVEIRA, G. C.; JUNQUEIRA, N. B.; MENOZZI, B. D.; JOAQUIM, S. F. Considerações sobre o tratamento das mastites. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 11, p. 1261-1269, novembro 2017. Título em inglês: Considerations on the treatment of mastitis.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | ATHAYDE, N. B.; DALLA COSTA, O. A.; ROÇA, R. de O; GUIDONI, A. L.; JOAQUIM, S. F.; HOMEM, J. M. Influência do uso de ractopamina na dieta de suínos em terminação sobre a concentração de lactato e cortisol sanguíneos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém. Anais... Belém: UFRA, 2011. 1 CD-ROM. Projeto: 02.06.01.001.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 4. | | GUIMARÃES, F. F.; JOAQUIM, S. F.; MANZI, M. P.; SILVA, R. C. da; BRUDER-NASCIMENTO, A. C. M. de O.; COSTA, E. O.; LANGONI, H. Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 12, p. 1155-1160-1164, dez. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | MANZI, M. P.; JOAQUIM, S. F.; GUIMARÃES, F. F.; BRUDER-NASCIMENTO, A. C. M. O.; PANTOJA, J. C. F.; LANGONI, H. Prevalência de Mycoplasma bovis em rebanhos de vacas leiteiras. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 665-669, abril 2018 Título em inglês: Prevalence of Mycoplasma bovis in dairy herds.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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